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An unopened vial should be used before the expiration date indicated by the manufacturer

Mechanisms of epigenetic modifications The epigenetic modifications can be generally categorized into three groups: DNA and RNA methylations, histone modifications, and non-coding RNAs, which are considered as main mechanisms of regulation during carcinogenesis/cancer progression

Hoechst staining Hoechst 33342 was used to specifically stain the nuclei of living and fixed cells and tissues, which allowed for the measurement of apoptosis within cells